Analysis of six autosomes
Sex chromosome analysis
Single tube reaction
Miscarriage is defined as the spontaneous loss of pregnancy before the fetus reaches viability and includes all pregnancy losses from the time of conception until 24 weeks of gestation. Recurrent miscarriage is the loss of three or more consecutive pregnancies, and affects nearly 1% of couples trying to conceive.
50% of first trimester recurrent miscarriage cases have been shown to be caused by a chromosome abnormality (primarily aneuploidy); the most commonly noted are trisomies, which account for 60% of all chromosome abnormalities in miscarriage.
The most frequent trisomy found in products of conception(POC) is trisomy for chromosome 16. However, trisomies for chromosomes 13, 15, 18, 21 and 22 are also common. Other aneuploidies commonly seen include monosomy X and triploidy which account for approximately 20% and 15% of all abnormalities, respectively.
QST*R-PL (Pregnancy Loss Kit)
The QST*R-PL Kit is for the routine in vitro quantitative diagnosis of the six most common autosomal trisomies associated with pregnancy loss: trisomy 13 (Patau syndrome), trisomy 15, trisomy 16, trisomy 18 (Edwards syndrome), trisomy 21 (Down syndrome) and trisomy 22. The kit also includes X and Y chromosome markers and the TAF9L marker for the determination of sex status. The results obtained from QST*R-PL Kit will determine the aneuploidy status of the fetus and may be useful in the management of the consequences resulting from the spontaneous miscarriage and for modification of risk calculations for future pregnancies.
- QST*R-PL contains 4 x STR markers for chromosomes 13, 15, 16, 18, 21, 22
- QST*R-PL contains X specific counting marker TAF9
- QST*R-PL contains X and Y specific markers AMEL and SRY
We have developed QST*R-PL for the fast, simple to use and accurate diagnosis of the six most common autosomal trisomies associated with pregnancy loss. QST*R-PL employs the commonly used QF-PCR (Quantitative Fluorescence-Polymerase Chain Reaction) technique, which has a number of advantages over other techniques for analyzing recurrent pregnancy loss.
- Simple to use method
- Low failure rates
- Fast 1 day sample to diagnosis
- Ability to identify maternal genome contamination
How does the QST*R-PL kit work?
Using PCR amplification, fluorescent dye labelled primers target highly polymorphic regions of DNA sequence called short tandem repeats (STRs) that are located on the chromosomes of interest. Amplified products of the QF-PCR technique are analyzed quantitatively on a capillary electrophoresis Genetic Analyzer to determine the copy number of the analyzed STR markers.
|Kit Name||Description||Platform||Catalogue Code|
Elucigene QST*R-PL Pregnancy Loss Kit
Detects trisomies of chromosome 13, 15, 16, 18, 21, 22, X and Y.
Elucigene kits are developed and manufactured within quality systems accredited to ISO9001:2008 and ISO13485:2003 and are validated as in vitro diagnostic devices in compliance with the European Community Directive 98/79/EC and the Canadian Medical Device Regulations (CMDR). Elucigene is a trademark of Delta Diagnostics (UK) Ltd. ARMS is a trademark of AstraZeneca UK Ltd. GeneMarker is a trademark of SoftGenetics Corporation. VIC®, PET® and NED™ are trademarks of Life Technologies Corporation.